Zearalenone degrading enzyme evolution to increase the hydrolysis efficiency under acidic conditions by the rational design.
Food Chem
; 456: 140088, 2024 Oct 30.
Article
em En
| MEDLINE
| ID: mdl-38878543
ABSTRACT
Based on rational design, zearalenone degrading enzyme was evolved to improve the hydrolysis efficiency under acidic conditions. At pH 4.2 and 37 °C, the activity of the zearalenone degrading enzyme evolved with 8 mutation sites increased from 7.69 U/mg to 38.67 U/mg. Km of the evolved zearalenone degrading enzyme decreased from 283.61 µM to 75.33 µM. The evolved zearalenone degrading enzyme was found to effectively degrade zearalenone in pig stomach chyme. Molecular docking revealed an increase in the number of hydrogen bonds and π-sigma interactions between the evolved zearalenone degrading enzyme and zearalenone. The evolved zearalenone degrading enzyme was valuable for hydrolyzing zearalenone under acidic conditions.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Zearalenona
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Simulação de Acoplamento Molecular
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article