Antibacterial Activity of the Vancomycin and Cefotaxime-Incorporated Total Etch Adhesive System - An <i>In Vitro</i> Study.

Rangareddy, M S; Wahed, Md Abdul; Kumar, B Shravan; Karteek, Basa Srinivas; Reddy, Chavva Lakshmi Charan; Agrawal, Jagrati

Antibacterial Activity of the Vancomycin and Cefotaxime-Incorporated Total Etch Adhesive System - An In Vitro Study.

Rangareddy, M S; Wahed, Md Abdul; Kumar, B Shravan; Karteek, Basa Srinivas; Reddy, Chavva Lakshmi Charan; Agrawal, Jagrati.

Afiliação

Rangareddy MS; Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Kaloji Narayana Rao University of Health Sciences, Warangal, Telangana, India.
Wahed MA; Department of Conservative Dentistry and Endodontics, Malla Reddy Institute of Dental Sciences, Kaloji Narayana Rao University of Health Sciences, Warangal, Telangana, India.
Kumar BS; Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Kaloji Narayana Rao University of Health Sciences, Warangal, Telangana, India.
Karteek BS; Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Kaloji Narayana Rao University of Health Sciences, Warangal, Telangana, India.
Reddy CLC; Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Kaloji Narayana Rao University of Health Sciences, Warangal, Telangana, India.
Agrawal J; Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Kaloji Narayana Rao University of Health Sciences, Warangal, Telangana, India.

J Pharm Bioallied Sci ; 16(Suppl 2): S1705-S1710, 2024 Apr.

Article em En | MEDLINE | ID: mdl-38882884

ABSTRACT

ABSTRACT

Background:

Marginal failure at the resin dentin interface promotes biofilm formation, which further leads to secondary caries and hypersensitivity. This likelihood also increases if residual bacteria are present following cavity preparation. In order to achieve a proper biological seal without jeopardizing bonding, efforts were made to functionalize the adhesive system with antibacterial activity. Aim and

objectives:

To appraise the antibacterial activity of a total-etch adhesive system against S. mutans with and without incorporation of antibiotics Vancomycin and Cefotaxime. Materials and

Method:

A commercially available 5th-generation total-etch bonding agent (Te-Econorm) was used. S. mutans broth had been standardized and streaked over Muller-Hinton agar culture medium and round wells about 6 mm in diameter were made in the centre of the agar plates. Each experimental group comprised 10 samples, which include Group 1 - 30µg Cefotaxime, Group 2- 30µg Cefotaxime + Bonding agent, Group 3- 30µg Vancomycin, Group 4- 30µg Vancomycin + Bonding agent, Group 5- Bonding agent, and Group 6- No material. Inoculated culture plates were examined for the zone of inhibition after incubation at 37° C for 24 hours.

Results:

There was a significant difference in the mean diameter of zone of inhibition (p=0.000), with the maximum exhibited by Group 4, followed by Group 3 and Group 2. The least zone of inhibition was exhibited by Groups 1 and 5. The negative control showed no zone of inhibition.