Exertional heat stress promotes the presence of bacterial DNA in plasma: A counterbalanced randomised controlled trial.
J Sci Med Sport
; 27(9): 610-617, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38906729
ABSTRACT
OBJECTIVES:
The primary aim was to explore the impact of exertional-heat stress (EHS) promoted exercise-associated bacteraemia. A secondary aim was to examine if an amino acid beverage (AAB) intervention may mitigate exercise-associated bacteraemia.DESIGN:
Counterbalanced randomised control trial.METHODS:
Twenty endurance trained male participants completed two randomised EHS trials. On one occasion, participants consumed a 237â¯mL AAB twice daily for 7â¯days prior, immediately before and every 20â¯min during EHS (2â¯h running at 60â¯% VÌO2max in 35⯰C). On the other occasion, a water volume control (CON) equivalent was consumed. Whole blood samples were collected pre- and immediately post-EHS, and were analysed for plasma DNA concentration by fluorometer quantification after microbial extraction, and bacterial relative abundance by next generation 16s rRNA gene sequencing.RESULTS:
Increased concentration of microbial DNA in plasma pre- to post-EHS was observed on CON (pre-EHS 0.014â¯ng/µL, post-EHS 0.039â¯ng/µL) (pâ¯<â¯0.001) and AAB (pre-EHS 0.015â¯ng/µL, post-EHS 0.031â¯ng/µL) (pâ¯<â¯0.001). The magnitude of change from pre- to post-exercise on AAB was 40â¯% lower, but no significant difference was observed versus CON (pâ¯=â¯0.455). Predominant bacterial groups identified included phyla-Proteobacteria (88.0â¯%), family-Burkholderiaceae (59.1â¯%), and genus-Curvibacter (58.6â¯%). No significant variation in absolute and relative change in α-diversity and relative abundance for phyla, family, and genus bacterial groups was observed in AAB versus CON.CONCLUSIONS:
The increased presence of microbial-bacterial DNA in systemic circulation in response to EHS appears positive in all participants. An amino acid beverage supplementation period prior to and consumption during EHS did not provide significant attenuation of EHS-associated bacteraemia.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article