Single-Cell Proteomics Analysis with Tecan Uno and SCREEN Workflow.
Methods Mol Biol
; 2817: 157-175, 2024.
Article
em En
| MEDLINE
| ID: mdl-38907154
ABSTRACT
With advances in sample preparation, small-volume liquid dispensing technologies, high-resolution MS/MS instrumentation, and data acquisition methodologies, it has become increasingly possible to confidently investigate the heterogeneous proteome found within individual cells. In this chapter, we present an automated high-throughput sample preparation workflow based on the Tecan Uno instrument for quantitative single-cell mass spectrometry-based proteomics. Cells are analyzed by the Single-Cell Proteome Analysis platform (SCREEN), which was introduced earlier and provides deeper proteome coverage across single cells.
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MEDLINE
Assunto principal:
Proteoma
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Proteômica
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Espectrometria de Massas em Tandem
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Fluxo de Trabalho
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Análise de Célula Única
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article