Assessing MicroRNA Profiles from Low Concentration Extracellular Vesicle RNA Utilizing NanoString nCounter Technology.
Methods Mol Biol
; 2822: 101-123, 2024.
Article
em En
| MEDLINE
| ID: mdl-38907915
ABSTRACT
Extracellular vesicles (EV) are rich in small RNA; however, a frequent caveat can be low abundance of EV RNA content, especially in clinical studies. NanoString MicroRNA Assays allow for multiplexed profiling of n = 800 mature microRNAs and can be applied to assess EV microRNA cargo. Here, we describe a method to adapt NanoString nCounter microRNA profiling to assess mature microRNA expression in low-concentration RNA samples, including concentrating the RNA, quantifying the RNA, and performing the NanoString protocol. Twelve samples can be assessed at one time using this method.
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Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Vesículas Extracelulares
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article