Multidimensional combinatorial screening for high-level production of erythritol in Yarrowia lipolytica.
Bioresour Technol
; 406: 131035, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38925409
ABSTRACT
Yarrowia lipolytica was successfully engineered to synthesize erythritol from crude glycerol, a cheap by-product of biodiesel production, but the yield remained low. Here, a biosensor-guided adaptive evolution screening platform was constructed to obtain mutant strains which could efficiently utilize crude glycerol to produce erythritol. Erythrose reductase D46A (M1) was identified as a key mutant through whole-genome sequencing of the strain G12, which exhibited higher catalytic activity (1.6-fold of the wild-type). M1 was further modified to obtain a combinatorial mutant with 4.1-fold enhancement of catalytic activity. Finally, the metabolic network was reconfigured to redirect carbon fluxes toward erythritol synthesis. The erythritol titer of the engineered strain G31 reached 220.5 g/L with a productivity of 1.8 g/L/h in a 5-L bioreactor. The study provides valuable guidance for biosensor-based ultra-high-throughput screening strategies in Y. lipolytica, as well as presenting a new paradigm for the sustainable valorization of crude glycerol.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Yarrowia
/
Eritritol
/
Glicerol
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article