A self-immobilizing near-infrared fluorogenic probe for in vivo imaging of fibroblast activation protein-α.
Talanta
; 278: 126475, 2024 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-38944939
ABSTRACT
Fibroblast activation protein-α (FAP) plays a crucial role in various physiological and pathological processes, making it a key target for cancer diagnostics and therapeutics. However, in vivo detection of FAP activity with fluorogenic probes remains challenging due to the rapid diffusion and clearance of fluorescent products from the target. Herein, we developed a self-immobilizing near-infrared (NIR) fluorogenic probe, Hcy-CF2H-PG, by introducing a difluoromethyl group to FAP substrate-caged NIR fluorophore. Upon selective activation by FAP, the fluorescence of Hcy-CF2H-PG was triggered, followed by the covalent labelling of FAP. Hcy-CF2H-PG demonstrated significantly improved sensitivity, selectivity, and long-lasting labelling capacity for FAP both in vitro and in vivo, compared to that of non-immobilized probes. This represents a noteworthy advancement in FAP detection and cancer diagnostics within complex physiological systems.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Serina Endopeptidases
/
Gelatinases
/
Corantes Fluorescentes
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article