H9N2 influenza A viruses found to be enzootic in Punjab Pakistan's bird markets with evidence of human H9N2 nasal colonization.
Int J Infect Dis
; 146: 107146, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38945434
ABSTRACT
OBJECTIVES:
This study sought to detect and characterize influenza A (IAV) and influenza D (IDV) viruses circulating among commercial birds and shop owners in Pakistan's live bird markets.METHODS:
Oropharyngeal swabs (n = 600; n = 300 pools) collected from poultry and nasopharyngeal swabs (n = 240) collected from poultry workers were studied for molecular evidence of IAV and IDV using real-time and conventional real-time reverse transcription polymerase chain reaction protocols.RESULTS:
Nineteen (6.3%) poultry pools were positive for IAV and 73.9% of these were positive for H9N2 subtypes. Two (0.83%) poultry workers had evidence of IAV, and both were also H9N2 subtypes. The poultry and human IAV-positive specimens all clustered phylogenetically by Sanger and next-generation sequencing with previously detected H9N2 poultry isolates. No field specimens were positive for IDV.CONCLUSION:
H9N2 IAV is likely enzootic in Punjab Province Pakistan's live bird markets and may be colonizing the noses of workers and market visitors. Regular monitoring for avian influenza-associated human illness in Punjab seems to be a needed public measure.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Aves Domésticas
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Influenza Humana
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Vírus da Influenza A Subtipo H9N2
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Influenza Aviária
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article