Effect of novel lyophilized extenders on stallion post-thaw sperm motility.
J Equine Vet Sci
; 140: 105144, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38945462
ABSTRACT
The aim of this study was to assess the effect of lyophilized freezing extenders, which can be stored at room temperature, on stallion post-thaw sperm total motility (TM). Ejaculates of 28 stallions were frozen with four different extenders two commercial freezing extenders offered worldwide and two novel lyophilized extenders (STAR and MX3), and two different cryopreservation protocols (CP1 with an equilibration period of 20 min. and CP2 with an equilibration period of 60 min.). The TM was assessed after thaw. Mean TM did not show significant differences between cryopreservation protocols within each extender. Mean TM was greater in samples diluted with STAR than in samples diluted with Botucrio (P Ë 0.05), but no significant differences were observed for this variable between the other studied extenders. From all evaluated samples, twenty ejaculates showed the greatest TM when using the lyophilized extenders and the CP1. Thus, lyophilized extenders are a promising option for stallion sperm cryopreservation and have the advantage of storage and distribution at room temperature for at least one year.
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MEDLINE
Assunto principal:
Preservação do Sêmen
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Motilidade dos Espermatozoides
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Criopreservação
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Crioprotetores
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Liofilização
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article