Optimized Workflow from Gene to Product.
Methods Mol Biol
; 2829: 49-66, 2024.
Article
em En
| MEDLINE
| ID: mdl-38951326
ABSTRACT
This chapter outlines the workflow using the ExpiSf™ Expression System designed for high-density infection of suspension ExpiSf9™ cells. The system utilizes a chemically defined, serum-free, protein-free, and animal origin free medium, making it suitable for recombinant protein expression experiments. The ExpiSf™ chemically defined medium allows efficient transfection and baculovirus production directly within the same culture medium. The ExpiSf™ Expression System Starter Kit provides all necessary components, including cells, culture medium, and reagents needed to infect one (1) liter of cell culture. The system's versatility and animal origin free nature make it a valuable tool for various protein expression studies and biotechnological applications.
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1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
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Baculoviridae
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Fluxo de Trabalho
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article