Recombinant AAV Purification.
Methods Mol Biol
; 2829: 217-226, 2024.
Article
em En
| MEDLINE
| ID: mdl-38951337
ABSTRACT
Purification of rAAV is a crucial unit operation of the AAV production process. It enables the capture of AAV and removal of contaminants such as host cell proteins, host cell DNA, and other cell culture-related impurities. Here we describe the purification of rAAV produced in insect cells Sf9/rBEV by immuno-affinity capture chromatography. The method is fully scale-amenable unlike other traditional purification methods based on ultracentrifugation. The method reported herein has two main steps:
(1) the clarification of cell lysate by depth filtration and (2) the selective capture and single-step purification of AAV via immune-affinity chromatography. This purification method has been successfully implemented to purify the majority of wild-type AAV serotypes.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Dependovirus
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article