hsp70 PCR-RFLP as an alternative tool to identify Sauroleishmania species.
Parasitol Res
; 123(7): 260, 2024 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-38958778
ABSTRACT
Sauroleishmania spp. comprises one of the four Leishmania subgenera, which has been historically considered a non-pathogenic protozoan of reptiles. However, some strains appear to be transiently infective to mammals, and recent findings have detected these parasites in dogs and humans in areas where leishmaniasis is endemic. Herein, the digestion pattern of PCR-RFLP of the 234 bp-hsp70 fragment was evaluated as a simpler and cheaper tool to distinguish the Sauroleishmania species from the other Leishmania subgenera. As a result, the digestion of the 234 bp-hsp70 fragments with HaeIII produced a banding pattern specific to the four Sauroleishmania strains assessed. This technique could contribute to the identification of Leishmania parasites isolated from sandflies, reptiles, or even mammals in fieldworks as an alternative to the use of laborious and expensive methodologies.
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
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Reação em Cadeia da Polimerase
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Proteínas de Choque Térmico HSP70
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Leishmania
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article