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Bacterial diversity and specific taxa are associated with decolonization of carbapenemase-producing enterobacterales after fecal microbiota transplantation.
Davido, Benjamin; Watson, Andrea R; de Truchis, Pierre; Galazzo, Gianluca; Dinh, Aurelien; Batista, Rui; Terveer, Elisabeth M; Lawrence, Christine; Michelon, Hugues; Jobard, Marion; Saleh-Mghir, Azzam; Kuijper, Ed J; Caballero, Silvia.
Afiliação
  • Davido B; Maladies Infectieuses, Hôpital Universitaire Raymond-Poincaré, AP-HP Université Paris Saclay, 92380 Garches, France; FHU PaCeMM, Hôpital Saint-Antoine, AP-HP Université Paris Centre, 75571 Paris Cedex 12, France. Electronic address: benjamin.davido@aphp.fr.
  • Watson AR; Vedanta Biosciences Inc., Cambridge, MA 02139, USA.
  • de Truchis P; Maladies Infectieuses, Hôpital Universitaire Raymond-Poincaré, AP-HP Université Paris Saclay, 92380 Garches, France.
  • Galazzo G; Vedanta Biosciences Inc., Cambridge, MA 02139, USA.
  • Dinh A; Maladies Infectieuses, Hôpital Universitaire Raymond-Poincaré, AP-HP Université Paris Saclay, 92380 Garches, France; FHU PaCeMM, Hôpital Saint-Antoine, AP-HP Université Paris Centre, 75571 Paris Cedex 12, France.
  • Batista R; Pharmacie Hospitalière, Hôpital Universitaire Cochin, AP-HP, 75014 Paris, France.
  • Terveer EM; Department of Medical Microbiology, Netherlands Donor Feces Bank (NDFB) at Leiden University Medical Center, Leiden, the Netherlands.
  • Lawrence C; Laboratoire de Microbiologie, Hôpital Universitaire Raymond-Poincaré, AP-HP Université Paris Saclay, 92380 Garches, France.
  • Michelon H; Pharmacie Hospitalière, Hôpital Universitaire Raymond-Poincaré, AP-HP Université Paris Saclay, 92380 Garches, France.
  • Jobard M; Pharmacie Hospitalière, Hôpital Universitaire Cochin, AP-HP, 75014 Paris, France.
  • Saleh-Mghir A; Maladies Infectieuses, Hôpital Universitaire Raymond-Poincaré, AP-HP Université Paris Saclay, 92380 Garches, France; UMR1173, Université Versailles Saint-Quentin, 78000 Versailles, France.
  • Kuijper EJ; Department of Medical Microbiology, Netherlands Donor Feces Bank (NDFB) at Leiden University Medical Center, Leiden, the Netherlands.
  • Caballero S; Vedanta Biosciences Inc., Cambridge, MA 02139, USA.
J Infect ; 89(2): 106216, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38964511
ABSTRACT

OBJECTIVES:

We evaluated the effect of fecal microbiota transplantation (FMT) on the clearance of carbapenemase-producing Enterobacterales (CPE) carriage.

METHODS:

We performed a prospective, multi-center study, conducted among patients who received a single dose of FMT from one of four healthy donors. The primary endpoint was complete clearance of CPE carriage two weeks after FMT with a secondary endpoint at three months. Shotgun metagenomic sequencing was performed to assess gut microbiota composition of donors and recipients before and after FMT.

RESULTS:

Twenty CPE-colonized patients were included in the study, where post-FMT 20% (n = 4/20) of patients met the primary endpoint and 40% (n = 8/20) of patients met the secondary endpoint. Kaplan-Meier curves between patients with FMT intervention and the control group (n = 82) revealed a similar rate of decolonization between groups. Microbiota composition analyses revealed that response to FMT was not donor-dependent. Responders had a significantly lower relative abundance of CPE species pre-FMT than non-responders, and 14 days post-FMT responders had significantly higher bacterial species richness and alpha diversity compared to non-responders (p < 0.05). Responder fecal samples were also enriched in specific species, with significantly higher relative abundances of Faecalibacterium prausnitzii, Parabacteroides distasonis, Collinsella aerofaciens, Alistipes finegoldii and Blautia_A sp900066335 (q<0.01) compared to non-responders.

CONCLUSION:

FMT administration using the proposed regimen did not achieve statistical significance for complete CPE decolonization but was correlated with the relative abundance of specific bacterial taxa, including CPE species.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fezes / Transplante de Microbiota Fecal / Microbioma Gastrointestinal Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fezes / Transplante de Microbiota Fecal / Microbioma Gastrointestinal Idioma: En Ano de publicação: 2024 Tipo de documento: Article