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Identification of circulating miRNA biomarkers in leptospirosis for early detection: A promising diagnostic approach.
Pinto, Gillaine Vail; Rai, Praveen; Kabekkodu, Shama Prakash; Karunasagar, Indrani; Kumar, Ballamoole Krishna.
Afiliação
  • Pinto GV; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India.
  • Rai P; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India.
  • Kabekkodu SP; Nitte (Deemed to Be University), Department of General Medicine, K.S. Hegde Medical Academy, Deralakatte, Mangalore, 575018, Karnataka, India.
  • Karunasagar I; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India.
  • Kumar BK; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India. Electronic address: krishnakumarb@nitte.edu.in.
Microb Pathog ; 193: 106781, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38969187
ABSTRACT
Leptospirosis is a zoonotic disease of global significance, contributing to morbidity and mortality worldwide. It is endemic to tropical regions, with outbreaks during monsoons. The disease manifestations are similar to that of other febrile illness such as dengue, malaria hence often misdiagnosed and underreported. The zoonoses if undetected, progresses to cause severe life-threatening complications also known as Weil's disease. Routine diagnostic tests are based on the detection of antibodies in patient serum and are not accurate during the initial phase of the infection. Therefore, it is necessary to detect novel biomarkers that can be used in early detection of leptospirosis. Circulating miRNAs are known to be promising biomarkers for various diseases including cancer, tuberculosis, influenza; hence in this study the potential of miRNAs as biomarkers for leptospirosis was evaluated. A total of 30 leptospirosis cases were screened for the differential expression of 10 miRNA by RT-qPCR assay. The differential expression was calculated by relative quantification using healthy individuals as controls. Among the 10 miRNA,3 miRNA, miR-28-5p, miR-302c-3p and miR-302a-3p were reported to exhibit a significant trend of upregulation. Further their role in immune pathways and biological processes was investigated by KEGG analysis and Gene Ontology. The 3 miRNAs were observed to target various immune response pathways, thus confirming their role in host immune response. Based on the results obtained in this study, miR-28-5p, miR-302c-3p and miR-302a-3p can be considered as potential biomarkers for the detection of leptospirosis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biomarcadores / Diagnóstico Precoce / MicroRNA Circulante / Leptospirose Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biomarcadores / Diagnóstico Precoce / MicroRNA Circulante / Leptospirose Idioma: En Ano de publicação: 2024 Tipo de documento: Article