Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy.
Methods Enzymol
; 700: 385-411, 2024.
Article
em En
| MEDLINE
| ID: mdl-38971608
ABSTRACT
Plasma membranes are flexible and can exhibit numerous shapes below the optical diffraction limit. The shape of cell periphery can either induce or be a product of local protein density changes, encoding numerous cellular functions. However, quantifying membrane curvature and the ensuing sorting of proteins in live cells remains technically demanding. Here, we demonstrate the use of simple widefield fluorescence microscopy to study the geometrical properties (i.e., radius, length, and number) of thin membrane protrusions. Importantly, the quantification of protrusion radius establishes a platform for studying the curvature preferences of membrane proteins.
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Base de dados:
MEDLINE
Assunto principal:
Transporte Proteico
/
Proteínas de Membrana
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Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article