Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling.
Methods Mol Biol
; 2816: 69-75, 2024.
Article
em En
| MEDLINE
| ID: mdl-38977589
ABSTRACT
Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we describe the protocols to measure intracellular Ca2+ for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Receptores Acoplados a Proteínas G
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article