Method to Assess the Intracellular Fate and Bioavailability of Clusterin Using Live Cell Confocal Microscopy Imaging.
Methods Mol Biol
; 2816: 145-149, 2024.
Article
em En
| MEDLINE
| ID: mdl-38977596
ABSTRACT
Clusterin, also known as apolipoprotein J, is an ATP-independent holdase chaperone protein. Clusterin is involved in various functions including protein quality control and lipid transport. Though clusterin is secreted upon stress, the intracellular fate of clusterin after a stress response is not well understood. The protocol described here utilizes clusterin tagged to fluorescent proteins like green fluorescent protein and red fluorescent protein to understand the intracellular fate of clusterin.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Microscopia Confocal
/
Clusterina
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article