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Association between Helicobacter pylori infection, mismatch repair, HER2 and tumor-infiltrating lymphocytes in gastric cancer.
Castaneda, Carlos A; Castillo, Miluska; Bernabe, Luis A; Sanchez, Joselyn; Fassan, Matteo; Tello, Katherine; Wistuba, Ignacio Ivan; Chavez Passiuri, Ivan; Ruiz, Eloy; Sanchez, Juvenal; Barreda, Fernando; Valdivia, Daniel; Bazan, Yaqueline; Abad-Licham, Milagros; Mengoa, Claudio; Fuentes, Hugo; Montenegro, Paola; Poquioma, Ebert; Alatrista, Raul; Flores, Claudio J; Taxa, Luis.
Afiliação
  • Castaneda CA; Faculty of Health Sciences, Universidad Cientifica del Sur, Lima 15038, Peru.
  • Castillo M; GECO PERU, Grupo de Estudios Clinicos Oncologicos del Peru, Lima 15038, Peru. ccastaneda@gecoperu.org.
  • Bernabe LA; Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Sanchez J; Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Fassan M; Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Tello K; Faculty of Human Medicine, Universidad Ricardo Palma, Lima 15039, Peru.
  • Wistuba II; Department of Medicine, Surgical Pathology & Cytopathology Unit, University of Padua, Padua 35121, Italy.
  • Chavez Passiuri I; Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Ruiz E; Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States.
  • Sanchez J; Department of Abdominal Surgery, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Barreda F; Department of Abdominal Surgery, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Valdivia D; Department of Pathology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Bazan Y; Department of Medical Specialties, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Abad-Licham M; Department of Medical Specialties, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Mengoa C; Department of Pathology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Fuentes H; Department of Pathology, Instituto Regional de Enfermedades Neoplasicas del Norte, Trujillo 13001, Peru.
  • Montenegro P; Faculty of Human Medicine, Universidad Privada Antenor Orrego, Trujillo 13008, Peru.
  • Poquioma E; Department of Surgery, Instituto Regional de Enfermedades Neoplasicas del Sur, Arequipa 04002, Peru.
  • Alatrista R; Department of Medical Oncology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Flores CJ; Department of Medical Oncology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
  • Taxa L; Department of Epidemiology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
World J Gastrointest Oncol ; 16(6): 2487-2503, 2024 Jun 15.
Article em En | MEDLINE | ID: mdl-38994161
ABSTRACT

BACKGROUND:

The influence of Helicobacter-pylori (H. pylori) infection and the characteristics of gastric cancer (GC) on tumor-infiltrating lymphocyte (TIL) levels has not been extensively studied. Analysis of infiltrating-immune-cell subtypes as well as survival is necessary to obtain comprehensive information.

AIM:

To determine the rates of deficient mismatch-repair (dMMR), HER2-status and H. pylori infection and their association with TIL levels in GC.

METHODS:

Samples from 503 resected GC tumors were included and TIL levels were evaluated following the international-TILs-working-group recommendations with assessment of the intratumoral (IT), stromal (ST) and invasive-border (IB) compartments. The density of CD3, CD8 and CD163 immune cells, and dMMR and HER2-status were determined by immunohistochemistry (IHC). H. pylori infection was evaluated by routine histology and quantitative PCR (qPCR) in a subset of samples.

RESULTS:

dMMR was found in 34.4%, HER2+ in 5% and H. pylori-positive in 55.7% of samples. High IT-TIL was associated with grade-3 (P = 0.038), while ST-TIL with grade-1 (P < 0.001), intestinal-histology (P < 0.001) and no-recurrence (P = 0.003). dMMR was associated with high TIL levels in the ST (P = 0.019) and IB (P = 0.01) compartments, and ST-CD3 (P = 0.049) and ST-CD8 (P = 0.05) densities. HER2- was associated with high IT-CD8 (P = 0.009). H. pylori-negative was associated with high IT-TIL levels (P = 0.009) when assessed by routine-histology, and with high TIL levels in the 3 compartments (P = 0.002-0.047) and CD8 density in the IT and ST compartments (P = 0.001) when assessed by qPCR. A longer overall survival was associated with low IT-CD163 (P = 0.003) and CD8/CD3 (P = 0.001 in IT and P = 0.002 in ST) and high IT-CD3 (P = 0.021), ST-CD3 (P = 0.003) and CD3/CD163 (P = 0.002).

CONCLUSION:

TIL levels were related to dMMR and H. pylori-negativity. Low CD8/CD3 and high CD163/CD3 were associated with lower recurrence and longer survival.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article