Maribellus mangrovi sp. nov., an iron-reducing bacterium isolated from mangrove sediment.
Int J Syst Evol Microbiol
; 74(7)2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38995174
ABSTRACT
A novel facultatively anaerobic and Gram-stain-negative bacterium, designated FJH33T, was isolated from mangrove sediment sampled in Zhangzhou, PR China. Cells of strain FJH33T were rod-shaped or slightly curved-shaped, with widths of 0.3-0.5 µm and lengths of 1.0-3.0 µm. Optimum growth of strain FJH33T occurred in the presence of 3â% NaCl (w/v), at 33â°C and at pH 7.0. Oxidase activity was negative, while catalase activity was positive. Its iron-reducing ability was determined. Based on 16S rRNA gene sequence similarity, strain FJH33T was most closely related to Maribellus luteus XSD2T (95.1â%), followed by Maribellus sediminis Y2-1-60T (95.0â%) and Maribellus maritimus 5E3T (94.9â%). Genome analysis of strains FJH33T and M. luteus XSD2T revealed low genome relatedness, with an average nucleotide identity value of 73.8% and a digital DNA-DNA hybridization value of 19.0%. Phylogenetic trees built from 16S rRNA genes and genome sequences showed that strain FJH33T represents a relatively independent phylogenetic lineage within the genus Maribellus. The major cellular fatty acids (≥10â%) were iso-C15ââ0 and C18ââ1 ω9c. The sole respiratory quinone was MK-7. The polar lipids consisted of phosphatidylethanolamine, diphosphatidylcholine, diphosphatidyglycerol and one unidentified lipid. The DNA G+C content was 41.4âmol%. Based on the integrated results of phylogenetic, physiological, biochemical and chemotaxonomic characterizations, we propose that strain FJH33T represents a novel species of the genus Maribellus, for which the name Maribellus mangrovi sp. nov. is proposed. The type strain is FJH33T (=KCTC 102210T=MCCC 1H01459T).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Composição de Bases
/
DNA Bacteriano
/
RNA Ribossômico 16S
/
Técnicas de Tipagem Bacteriana
/
Análise de Sequência de DNA
/
Sedimentos Geológicos
/
Vitamina K 2
/
Ácidos Graxos
/
Hibridização de Ácido Nucleico
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article