Graphene oxide-functionalized molecular beacon for real-time interference-free detection of Ki-67 mRNA in living cells.
Talanta
; 278: 126538, 2024 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-39002264
ABSTRACT
Molecular beacons (MBs) based on hairpin-shaped oligonucleotides are captivating owing to their capability to enable effective real-time detection of cytosolic mRNA in living cells. However, DNase in the nucleus and lysosome could induce the degradation of oligonucleotides in MBs, leading to the generation of false-positive signals. Herein, a graphene oxide (GO) nanosheet was applied as a nanocarrier for MBs to greatly enhance the anti-interference of the easily designed nanoprobe. Advantageously, the absorption capacity of GO for MBs increased with the decrease in pH values, providing the MB-GO nanoprobe with the ability to detect the expression of cytosolic Ki-67 mRNA without interference from DNase â
¡ in lysosomes. Moreover, the size of GO nanosheets was considerably higher than that of the nuclear pore complex (NPC), which prevented nanoprobes from transition through the NPCs, thereby avoiding the generation of false-positive signals in the nucleus. Altogether, the present work affords a convenient approach for the successful detection of Ki-67 mRNA expression in the cytosol without interference from DNase â
/â
¡ in the nucleus/lysosome, which may be potentially further applied for the detection of other cytosolic RNAs.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Antígeno Ki-67
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Grafite
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article