Acceleration of chondrogenic differentiation utilizing biphasic core-shell alginate sulfate electrospun nanofibrous scaffold.
Colloids Surf B Biointerfaces
; 242: 114080, 2024 Oct.
Article
em En
| MEDLINE
| ID: mdl-39003847
ABSTRACT
Engineering new biomedical materials with tailored physicochemical, mechanical and biological virtues in order to differentiate stem cells into chondrocytes for cartilage regeneration has garnered much scientific interest. In this study, core/shell nanofibrous scaffold based on poly(É-caprolactone) (PCL) as a core material and alginate sulfate (AlgS)-poly(vinyl alcohol) (PVA) blend as shell materials (AlgS-PVA/PCL) was fabricated by emulsion electrospinning. In this vein, the influence of AlgS to PVA ratio (3070, 5050), organic to aqueous phase ratio (12, 13 and 15) and acid concentration (0, 10, 20, 30, 40 and 50â¯%) on nanofibers morphology were investigated. SEM images depicted that AlgS to PVA ratio of 3070 and 5050, organic to aqueous phase ratio of 13 and 15 and acid concentration of 30â¯% led to uniform, bead-free fibrous mats. AlgS-PVA/PCL scaffolds with AlgS to PVA ratio of 3070 and organic to aqueous phase ratio of 13, showed admirable mechanical features, high porosity (>90â¯%) with desirable swelling ratio in wet condition. In vitro assays indicated that the AlgS-PVA/PCL scaffold surface had desirable interaction with stem cells and promotes cells attachment, proliferation and differentiation. Thus, we envision that this salient structure could be an intriguing construction as a cartilage tissue-engineered scaffold.
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MEDLINE
Assunto principal:
Poliésteres
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Diferenciação Celular
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Condrogênese
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Engenharia Tecidual
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Alginatos
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Alicerces Teciduais
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Nanofibras
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article