Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis.
Autoimmunity
; 57(1): 2361749, 2024 Dec.
Article
em En
| MEDLINE
| ID: mdl-39007896
ABSTRACT
BACKGROUND:
Dysregulated circular RNAs (circRNAs) are involved in osteoarthritis (OA) progression.OBJECTIVE:
We aimed to explore the effect of hsa_circ_0044719 (circTRIM25) on the ferroptosis of chondrocytes.METHODS:
Chondrocytes were treated with interleukin (IL)-1ß to generate cell model. Cellular behaviours were measured using cell counting kit-8, enzyme-linked immunosorbent assay, relevant kits, propidium iodide staining, and immunofluorescence assay. Quantitative real-time polymerase chain reaction was performed to examine the expression of circTRIM25, miR-138-5p, and cAMP responsive element binding protein 1 (CREB1), and their interactions were assessed using luciferase reporter analysis and RNA pull-down assay.RESULTS:
CircTRIM25 was upregulated in OA tissues and IL-1ß-stimulated chondrocytes. Knockdown of circTRIM25 facilitated the viability and suppressed ferroptosis and inflammation of IL-1ß-induced cells. CircTRIM25 served as a sponge of miR-138-5p, which directly targets CREB1. Downregulation of miR-138-5p abrogated the effect induced by knockdown of circTRIM25. Furthermore, enforced CREB1 reversed the miR-138-5p induced effect. Moreover, knockdown of circTRIM25 attenuated cartilage injury in vivo.CONCLUSION:
Silencing of circTRIM25 inhibited ferroptosis of chondrocytes via the miR-138-5p/CREB axis and thus attenuated OA progression.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteoartrite
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Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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Condrócitos
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Condrogênese
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MicroRNAs
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RNA Circular
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article