Recombinant Biogenesis and Analysis of Cytochrome c Species.
Methods Mol Biol
; 2839: 195-211, 2024.
Article
em En
| MEDLINE
| ID: mdl-39008254
ABSTRACT
Recombinant expression and biogenesis of cytochrome c species is a simple and efficient method for the production of holocytochrome c species, thus presenting an avenue for the study of cytochrome c or the cytochrome c biogenesis pathways responsible for heme attachment. Here, we describe a method for recombinant E. coli production of holocytochrome c utilizing the System I (CcmABCDEFGH) bacterial cytochrome c biogenesis pathway, followed by analysis of cytochrome c species by cell lysis and heme stain.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
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Citocromos c
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Escherichia coli
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Heme
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article