CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization.
Res Sq
; 2024 Jul 02.
Article
em En
| MEDLINE
| ID: mdl-39011111
ABSTRACT
CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. We demonstrate that vitreous ice may be produced by plunging clipped grids with purified proteins into liquid ethane and that clipped grids may be reused several times for different protein samples. Furthermore, we demonstrate the vitrification of thin areas of cells prepared on gold-coated, pre-clipped grids.
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MEDLINE
Idioma:
En
Ano de publicação:
2024
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Article