High-Throughput Mapping of Chromosomal Conformations in E. coli Under Physiological Conditions Using Massively Multiplexed Mu Transposition.
Methods Mol Biol
; 2819: 125-146, 2024.
Article
em En
| MEDLINE
| ID: mdl-39028505
ABSTRACT
Many approaches for measuring three-dimensional chromosomal conformations rely upon formaldehyde crosslinking followed by subsequent proximity ligation, a family of methods exemplified by 3C, Hi-C, etc. Here we provide an alternative crosslinking-free procedure for high-throughput identification of long-range contacts in the chromosomes of enterobacteria, making use of contact-dependent transposition of phage Mu to identify distant loci in close contact. The procedure described here will suffice to provide a comprehensive map of transposition frequencies between tens of thousands of loci in a bacterial genome, with the resolution limited by the diversity of the insertion site library used and the sequencing depth applied.
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Base de dados:
MEDLINE
Assunto principal:
Mapeamento Cromossômico
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Cromossomos Bacterianos
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Escherichia coli
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article