RNA-Seq Analysis of Mammalian Prion Disease.
Methods Mol Biol
; 2812: 367-377, 2024.
Article
em En
| MEDLINE
| ID: mdl-39068373
ABSTRACT
A protein, which can attain a prion state, differs from standard proteins in terms of structural conformation and aggregation propensity. High-throughput sequencing technology provides an opportunity to gain insight into the prion disease condition when coupled with single-cell RNA-Seq analysis to reveal transcriptional changes during prion-based pathogenicity. In this chapter, we present a protocol for RNA-Seq analysis of mammalian prion disease using a single-cell RNA sequencing dataset procured from the NCBI GEO database. This protocol is a tool that can assist researchers in characterizing mammalian prion disease in a reproducible and reusable manner. Further, the resulting output has the potential to provide transcript biomarkers for mammalian prion diseases, which can be employed for diagnostic and prognostic purposes.
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Base de dados:
MEDLINE
Assunto principal:
Doenças Priônicas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article