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Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review-part II.
Goswami, Neha; Anastasio, Mark A; Popescu, Gabriel.
Afiliação
  • Goswami N; University of Illinois Urbana-Champaign, Department of Bioengineering, Urbana, Illinois, United States.
  • Anastasio MA; University of Illinois Urbana-Champaign, Department of Bioengineering, Urbana, Illinois, United States.
  • Popescu G; University of Illinois Urbana-Champaign, Department of Electrical and Computer Engineering, Urbana, Illinois, United States.
J Biomed Opt ; 29(Suppl 2): S22714, 2024 Jun.
Article em En | MEDLINE | ID: mdl-39070593
ABSTRACT

Significance:

Quantitative phase imaging (QPI) is a non-invasive, label-free technique that provides intrinsic information about the sample under study. Such information includes the structure, function, and dynamics of the sample. QPI overcomes the limitations of conventional fluorescence microscopy in terms of phototoxicity to the sample and photobleaching of the fluorophore. As such, the application of QPI in estimating the three-dimensional (3D) structure and dynamics is well-suited for a range of samples from intracellular organelles to highly scattering multicellular samples while allowing for longer observation windows.

Aim:

We aim to provide a comprehensive review of 3D QPI and related phase-based measurement techniques along with a discussion of methods for the estimation of sample dynamics.

Approach:

We present information collected from 106 publications that cover the theoretical description of 3D light scattering and the implementation of related measurement techniques for the study of the structure and dynamics of the sample. We conclude with a discussion of the applications of the reviewed techniques in the biomedical field.

Results:

QPI has been successfully applied to 3D sample imaging. The scattering-based contrast provides measurements of intrinsic quantities of the sample that are indicative of disease state, stage of growth, or overall dynamics.

Conclusions:

We reviewed state-of-the-art QPI techniques for 3D imaging and dynamics estimation of biological samples. Both theoretical and experimental aspects of various techniques were discussed. We also presented the applications of the discussed techniques as applied to biomedicine and biology research.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espalhamento de Radiação / Imageamento Tridimensional Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espalhamento de Radiação / Imageamento Tridimensional Idioma: En Ano de publicação: 2024 Tipo de documento: Article