Bacterial nanocellulose by static, static intermittent fed-batch and rotary disc bioreactor-based fermentation routes using economical black tea broth medium: A comparative account.

ABSTRACT

Bacterial nanocellulose was produced here using static, static intermittent-fed batch (SIFB) and rotary disc bioreactor (RDB) mode. Economical black tea broth media with symbiotic consortia of bacteria and yeast (SCOBY) was used towards feasible BNC production (instead of commercial NCIM 2526 strain and conventional HS media). The physicochemical characterization of BNC produced in all three modes via FE-SEM, ATR-FTIR, XRD and TGA results showed a highly porous morphology, mostly Iα form, good crystallinity and thermal stability, respectively. BNC crystallinity lies in the range of 68 % (RDB) to 79.4 % (static and SIFB). Water retention value (86 to 93 %) and moisture content (85 to 93 %) are high for BNC produced in all three modes. Commendable difference in the BNC yield, sugar consumption, conversion yield and residual sugar was observed using different methods. Highest BNC yield 29.4 ± 0.66 gL-1 was obtained under SIFB method as compared to static mode (13.6 ± 0.32 g L-1). Under RDB, a negligible amount of BNC i.e., 1.0 ± 0.2 g L-1 was produced. SCOBY with BTB medium was found unsuitable for BNC production under RDB and needs further investigation. Thus, this comparative study offers a way to produce a commendable amount of low-priced BNC for various techno-industrial usage.