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Hybrid immunity to SARS-CoV-2 arises from serological recall of IgG antibodies distinctly imprinted by infection or vaccination.
Voss, William N; Mallory, Michael A; Byrne, Patrick O; Marchioni, Jeffrey M; Knudson, Sean A; Powers, John M; Leist, Sarah R; Dadonaite, Bernadeta; Townsend, Douglas R; Kain, Jessica; Huang, Yimin; Satterwhite, Ed; Castillo, Izabella N; Mattocks, Melissa; Paresi, Chelsea; Munt, Jennifer E; Scobey, Trevor; Seeger, Allison; Premkumar, Lakshmanane; Bloom, Jesse D; Georgiou, George; McLellan, Jason S; Baric, Ralph S; Lavinder, Jason J; Ippolito, Gregory C.
Afiliação
  • Voss WN; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Mallory MA; Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Byrne PO; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Marchioni JM; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Knudson SA; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Powers JM; Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Leist SR; Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Dadonaite B; Basic Sciences Division and Computational Biology Program, Fred Hutchinson Cancer Center, Seattle, WA, USA.
  • Townsend DR; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Kain J; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Huang Y; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Satterwhite E; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Castillo IN; Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Mattocks M; Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Paresi C; Department of Chemistry, The University of Texas at Austin, Austin, TX, USA.
  • Munt JE; Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Scobey T; Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Seeger A; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • Premkumar L; Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Bloom JD; Basic Sciences Division and Computational Biology Program, Fred Hutchinson Cancer Center, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA, USA.
  • Georgiou G; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
  • McLellan JS; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
  • Baric RS; Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Lavinder JJ; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA. Electronic address: jlavinder@utexas.edu.
  • Ippolito GC; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA. Electronic address: gippolito@txbiomed.org.
Cell Rep Med ; : 101668, 2024 Jul 25.
Article em En | MEDLINE | ID: mdl-39094579
ABSTRACT
We describe the molecular-level composition of polyclonal immunoglobulin G (IgG) anti-spike antibodies from ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, vaccination, or their combination ("hybrid immunity") at monoclonal resolution. Infection primarily triggers S2/N-terminal domain (NTD)-reactive antibodies, whereas vaccination mainly induces anti-receptor-binding domain (RBD) antibodies. This imprint persists after secondary exposures wherein >60% of ensuing hybrid immunity derives from the original IgG pool. Monoclonal constituents of the original IgG pool can increase breadth, affinity, and prevalence upon secondary exposures, as exemplified by the plasma antibody SC27. Following a breakthrough infection, vaccine-induced SC27 gained neutralization breadth and potency against SARS-CoV-2 variants and zoonotic viruses (half-maximal inhibitory concentration [IC50] ∼0.1-1.75 nM) and increased its binding affinity to the protective RBD class 1/4 epitope (dissociation constant [KD] < 5 pM). According to polyclonal escape analysis, SC27-like binding patterns are common in SARS-CoV-2 hybrid immunity. Our findings provide a detailed molecular definition of immunological imprinting and show that vaccination can produce class 1/4 (SC27-like) IgG antibodies circulating in the blood.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article