Antimicrobial resistance induction potential of grapefruit seed extract on multi-species biofilm of E. coli in food industry.
Int J Food Microbiol
; 424: 110849, 2024 Nov 02.
Article
em En
| MEDLINE
| ID: mdl-39098160
ABSTRACT
Biofilm formation in natural environments involving complex multi-structural arrangements hinders challenges in antimicrobial resistance. This study investigated the antimicrobial resistance potential of grapefruit seed extract (GSE) by examining the formation of mono-, dual-, and multi-species biofilms. We also explored the counterintuitive effect in response to GSE at various concentrations, including minimum inhibitory concentration (MIC) and sub-MIC (1/2 and 1/4 MIC). The results of the swimming and swarming motility tests revealed increased motility at the sub-MIC of GSE. The crystal violet assay demonstrated increased biofilm formation in multi-species biofilms, highlighting the synergistic effect of Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes. At the MIC concentration of GSE, field emission scanning electron microscopy (FE-SEM) revealed cell morphology damage, while sub-MIC increased biofilm formation and architectural complexity. Multi-species biofilms demonstrated greater biofilm-forming ability and antimicrobial resistance than mono-species biofilms, indicating synergistic interactions and enhanced resilience. These findings highlight the importance of understanding biofilm dynamics and antimicrobial resistance to ensure environmental safety.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sementes
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Extratos Vegetais
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Testes de Sensibilidade Microbiana
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Biofilmes
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Citrus paradisi
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Escherichia coli
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Listeria monocytogenes
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Antibacterianos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article