In vitrodegradation of a chitosan-based osteochondral construct points to a transient effect on cellular viability.
Biomed Mater
; 19(5)2024 Aug 06.
Article
em En
| MEDLINE
| ID: mdl-39105245
ABSTRACT
Bioresorbable chitosan scaffolds have shown potential for osteochondral repair applications. Thein vivodegradation of chitosan, mediated by lysozyme and releasing glucosamine, enables progressive replacement by ingrowing tissue. Here the degradation process of a chitosan-nHA based bioresorbable scaffold was investigated for mass loss, mechanical properties and degradation products released from the scaffold when subjected to clinically relevant enzyme concentrations. The scaffold showed accelerated mass loss during the early stages of degradation but without substantial reduction in mechanical strength or structure deterioration. Although not cytotoxic, the medium in which the scaffold was degraded for over 2 weeks showed a transient decrease in mesenchymal stem cell viability, and the main degradation product (glucosamine) demonstrated a possible adverse effect on viability when added at its peak concentration. This study has implications for the design and biomedical application of chitosan scaffolds, underlining the importance of modelling degradation products to determine suitability for clinical translation.
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Base de dados:
MEDLINE
Assunto principal:
Teste de Materiais
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Sobrevivência Celular
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Engenharia Tecidual
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Quitosana
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Alicerces Teciduais
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Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article