Generation of Directly Reprogrammed Human Endothelial Cells.
Methods Mol Biol
; 2835: 155-164, 2024.
Article
em En
| MEDLINE
| ID: mdl-39105914
ABSTRACT
Direct reprogramming provides a novel breakthrough for generating functional endothelial cells (ECs) without the need for intermediate stem or progenitor states, offering a promising resource for cardiovascular research and treatment. ETV2 is a key transcription factor that has been identified as a pioneering factor for specifying endothelial lineage. Achieving precise ETV2 induction is essential for effective endothelial reprogramming, and maintaining the reprogrammed cellular phenotype relies on a specific combination of growth factors and small molecules. Thus, we hereby provide a straightforward and comprehensive protocol for generating two distinct types of reprogrammed ECs (rECs) from human dermal fibroblasts (HDFs). Early rECs demonstrate a robust neovascularization property but lack the mature EC phenotype, while late rECs exhibit phenotypical similarity to human postnatal ECs and have a neovascularization capacity similar to early rECs. Both cell types can be derived from human somatic source cells, making them suitable for personalized disease investigations, drug discovery, and disease therapy.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
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Células Endoteliais
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Reprogramação Celular
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Fibroblastos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article