Variation in phytotoxicity of rice seedlings caused by differential accumulation of azoxystrobin and pyraclostrobin in leaves.

ABSTRACT

The effectiveness of pyraclostrobin (Pyr) and azoxystrobin (Azo) with highly targeting the rice blast is noteworthy, but they have varied toxic levels towards non-target aquatic organisms. Nevertheless, the toxic selectivity and mechanism of non-target plants, specifically rice, remain uncertain. In this study, we investigated the potential phytotoxic effects of Pyr and Azo on rice seedlings, including plant morphology, plant growth, physiological and biochemical changes. The findings revealed that both Pyr and Azo caused toxic effects on rice, resulting in symptoms of chlorosis and inhibited growth. The toxicity of Azo was found to be more severe when applied at the recommended field dose. Disruption of oxidative stress could significantly impact the demonstrated levels of REC, leading to a decrease in photosynthetic pigments and potentially culminating in cell death. Furthermore, the toxic effect of Azo had a greater impact on rice leaves compared to Pyr at treatments of 400, 800, 1600, and 4000 mg/L. However, the in vitro cytotoxicity of Azo on rice leaves was lower than that of Pyr. Therefore, it can be inferred that the mechanism of phytotoxicity of Azo is directly linked to the increased accumulation of the compound on the leaf tips and edges. Additionally, the positive effects observed on plant morphology and growth parameters suggest that the mixed application of plant growth regulators (sodium nitrophenolate aqueous solution of 14 mg/L and diethyl aminoethyl hexanoat of 50 mg/L) can be a promising approach to mitigate the rice phytotoxicity of Azo at 400 and 800 mg/L.