Structural characteristics and biological activity of a water-soluble polysaccharide HDCP-2 from Camellia sinensis.
Int J Biol Macromol
; 277(Pt 4): 134437, 2024 Aug 06.
Article
em En
| MEDLINE
| ID: mdl-39116965
ABSTRACT
Large-leaf Yellow tea (LYT) is a traditional beverage from Camellia Sinensis (L.) O. Kuntze in China and has unusual health-regulating functions. This investigation explored the structural characteristics of a polysaccharide extracted from LYT, which possesses anti-inflammatory activity. The polysaccharide HDCP-2, obtained through ethanol fractional precipitation and then DEAE-52 anion exchange column, followed by DPPH radical scavenging screening, exhibited a yield of 0.19 %. The HPGPC method indicated that the molecular weight of HDCP-2 is approximately 2.9 × 104 Da. Analysis of the monosaccharide composition revealed that HDCP-2 consisted of mannose, glucose, xylose, and galacturonic acid, and their molar ratio is approximately 0.40.51.20.7. The structure motif of HDCP-2 was probed carefully through methylation analysis, FT-IR, and NMR analysis, which identified the presence of ß-d-Xylp(1â, â2, 4)-ß-d-Xylp(1â, â3)-ß-d-Manp(1â, α-d-Glcp(1â and â2, 4)-α-d-GalAp(1â linkages. A CCK-8 kit assay was employed to evaluate the anti-inflammatory action of HDCP-2. These results demonstrated that HDCP-2 could inhibit the migration and proliferation of the MH7A cells and reduce NO production in an inflammatory model induced by TNF-α. The abundant presence of xylose accounted for 39 % of the LYT polysaccharide structure, and its distinctive linking mode (â2, 4)-ß-d-Xylp(1â) appears to be the primary contributing factor to its anti-inflammatory effect.
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MEDLINE
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Ano de publicação:
2024
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Article