SPP1 promotes the polarization of M2 macrophages through the Jak2/Stat3 signaling pathway and accelerates the progression of idiopathic pulmonary fibrosis.
Int J Mol Med
; 54(4)2024 Oct.
Article
em En
| MEDLINE
| ID: mdl-39129313
ABSTRACT
Idiopathic pulmonary fibrosis (IPF) is a fatal pulmonary disease that requires further investigation to understand its pathogenesis. The present study demonstrated that secreted phosphoprotein 1 (SPP1) was aberrantly highly expressed in the lung tissue of patients with IPF and was significantly positively associated with macrophage and Tcell activity. Cell localization studies revealed that SPP1 was primarily overexpressed in macrophages, rather than in T cells. Functionally, knocking down SPP1 expression in vitro inhibited the secretion of fibrosisrelated factors and M2 polarization in macrophages. Furthermore, knocking down SPP1 expression inhibited the macrophageinduced epithelialtomesenchymal transition in both epithelial and fibroblastic cells. Treatment with SPP1 inhibitors in vivo enhanced lung function and ameliorated pulmonary fibrosis. Mechanistically, SPP1 appears to promote macrophage M2 polarization by regulating the JAK/STAT3 signaling pathway both in vitro and in vivo. In summary, the present study found that SPP1 promotes M2 polarization of macrophages through the JAK2/STAT3 signaling pathway, thereby accelerating the progression of IPF. Inhibition of SPP1 expression in vivo can effectively alleviate the development of IPF, indicating that SPP1 in macrophages may be a potential therapeutic target for IPF.
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MEDLINE
Assunto principal:
Transdução de Sinais
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Fator de Transcrição STAT3
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Janus Quinase 2
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Osteopontina
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Fibrose Pulmonar Idiopática
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Macrófagos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article