A thermoresponsive chitosan-based in situ gel formulation incorporated with 5-FU loaded nanoerythrosomes for fibrosarcoma local chemotherapy.
Int J Biol Macromol
; : 134781, 2024 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-39151860
ABSTRACT
Local administration of drugs at tumor sites over an extended period of time shows potential as a promising approach for cancer treatment. In the present study, the temperature-induced phase transition of chitosan and poloxamer 407 is used to construct an injectable hydrogel encapsulating 5-FU-loaded nanoerythrosome (5-FU-NER-gel). The 5-FU-NERs were found to be spherical, measuring approximately 115⯱â¯20â¯nm in diameter and having a surface potential of -7.06⯱â¯0.4. The drug loading efficiency was approximately 40â¯%. In situ gel formation took place within 15â¯s when the gel was exposed to body temperature or subcutaneous injection. A sustained release profile was observed at pHâ¯7.4 and 6.8, with a total 5-FU release of 76.57⯱â¯4.4 and 98.07⯱â¯6.31 in 24â¯h, respectively. MTT, Live/Dead, and migration assays confirmed the cytocompatibility of the drug carrier and its effectiveness as a chemotherapeutic formulation. After in vivo antitumor assessment in a subcutaneous autograft model, it was demonstrated that tumor growth inhibition in 14â¯days was 90â¯%. Therefore, the obtained injectable chitosan-based hydrogel containing 5-FU-loaded nanoerythrosomes illustrated promising potential as a candidate for local and enhanced delivery of chemotherapeutics at the tumor site.
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MEDLINE
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En
Ano de publicação:
2024
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Article