Metamaterial-Assisted Illumination Nanoscopy with Exceptional Axial Resolution.
Adv Sci (Weinh)
; : e2404883, 2024 Aug 20.
Article
em En
| MEDLINE
| ID: mdl-39162105
ABSTRACT
Recent advancements in optical metamaterials have opened new possibilities in the exciting field of super-resolution microscopies. The far-field metamaterial-assisted illumination nanoscopies (MAINs) have, very recently, enhanced the lateral resolution to one-fifteenth of the optical wavelength. However, the axial localization accuracy of fluorophores in the MAINs remains rarely explored. Here, a MAIN with a nanometer-scale axial localization accuracy is demonstrated by monitoring the distance-dependent photobleaching dynamics of the fluorophores on top of an organic hyperbolic metamaterial (OHM) substrate under a wide-field single-objective microscope. With such a regular experimental configuration, 3D imaging of various biological samples with the resolution of ≈40 nm in the lateral dimensions and ≈5 nm in the axial dimension is realized. The demonstrated imaging modality enables the resolution of the 3D morphology of nanoscopic cellular structures with a significantly simplified experimental setup.
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MEDLINE
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En
Ano de publicação:
2024
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Article