[The role of dopamine receptors in the modulation of mononuclear phagocytes in multiple sclerosis]. / Rol' retseptorov dofamina v modulyatsii mononuklearnykh fagotsitov pri rasseyannom skleroze.
Zh Nevrol Psikhiatr Im S S Korsakova
; 124(7. Vyp. 2): 79-84, 2024.
Article
em Ru
| MEDLINE
| ID: mdl-39175244
ABSTRACT
OBJECTIVE:
To investigate the role of dopamine receptor D1DR and D2DR in the production of cytokines interleukin-6 (IL-6) and IL-1ß by monocytes and macrophages in patients with relapsing-remitting multiple sclerosis (MS). MATERIAL ANDMETHODS:
Ten patients with relapsing-remitting MS and 10 healthy subjects were examined. The level of IL-6 and IL-1ß production was assessed in culture supernatants obtained from CD14+ monocytes or macrophages stimulated with interferon-γ (IFN-γ) and lipopolysaccharide (LPS). To study the role of dopamine receptors in the regulation of CD14+ monocytes or macrophages, samples of cells were incubated in the presence of specific D1DR or D2DR antagonists, after which IFN-γ/LPS were added to the cultures. Levels of cytokines in culture supernatants were measured by enzyme-linked immunosorbent assay.RESULTS:
The production of IL-6 and IL-1ß by CD14+ monocytes and macrophages was comparable between the groups. Blockade of D1DR suppressed cytokine production by CD14+ monocytes and macrophages in both groups. In contrast, blockade of D2DR increased the production of cytokines by CD14+ monocytes and did not affect cytokine production by macrophages in both groups.CONCLUSIONS:
Targeting of dopaminergic receptors could be considered as an additional mechanism of immunomodulation in MS with both pro- and anti-inflammatory effects on cells of the innate immune system.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interleucina-6
/
Receptores de Dopamina D1
/
Esclerose Múltipla Recidivante-Remitente
/
Interleucina-1beta
/
Macrófagos
Idioma:
Ru
Ano de publicação:
2024
Tipo de documento:
Article