Cryo-Electron Microscopy in the Study of Antiviral Innate Immunity.
Methods Mol Biol
; 2854: 177-188, 2025.
Article
em En
| MEDLINE
| ID: mdl-39192129
ABSTRACT
Cryo-electron microscopy is a powerful methodology in structural biology and has been broadly used in high-resolution structure determination for challenging samples, which are not readily available for traditional techniques. In particular, the strength of super macro-complexes and the lack of a need for crystals for cryo-EM make this technique feasible for the structural study of complexes involved in antiviral innate immunity. This chapter presents detailed information and experimental procedures of Cryo-EM for determining the structures of the complexes using STING as an example. The procedures included a sample quality check, high-resolution data acquisition, and image processing for Cryo-EM 3D structure determination.
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Base de dados:
MEDLINE
Assunto principal:
Microscopia Crioeletrônica
/
Imunidade Inata
Idioma:
En
Ano de publicação:
2025
Tipo de documento:
Article