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Development of an AI-Assisted Embryo Selection System Using Iberian Ribbed Newts for Embryo-Fetal Development Toxicity Testing.
Saiki, Naofumi; Adachi, Akiko; Ohnishi, Hiroshi; Koga, Atsuro; Ueki, Masaru; Kohno, Kiyotaka; Hayashi, Toshinori; Ohbayashi, Tetsuya.
Afiliação
  • Saiki N; Division of Medical Education, Department of Medical Education, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
  • Adachi A; Advanced Medicine & Translational Research Center, Organization for Research Initiative and Promotion, Tottori University, Yonago 683-8503, Japan.
  • Ohnishi H; Technical Department, Tottori University, Yonago 683-8503, Japan.
  • Koga A; Advanced Medicine & Translational Research Center, Organization for Research Initiative and Promotion, Tottori University, Yonago 683-8503, Japan.
  • Ueki M; Division of Medical Education, Department of Medical Education, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
  • Kohno K; National Institute of Technology, Yonago College, Yonago 683-8502, Japan.
  • Hayashi T; Amphibian Research Center, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
  • Ohbayashi T; Advanced Medicine & Translational Research Center, Organization for Research Initiative and Promotion, Tottori University, Yonago 683-8503, Japan.
Yonago Acta Med ; 67(3): 233-241, 2024 Aug.
Article em En | MEDLINE | ID: mdl-39193136
ABSTRACT

Background:

The 3Rs (Reduction, Refinement, Replacement) principle is driving the need for alternative methods in animal testing. Despite advancements in in vitro testing, complex systemic toxicity tests still necessitate in vivo approaches. The aim of this study was to develop a developmental toxicity test protocol using the Iberian ribbed newt (Pleurodeles waltl) as a model organism, integrating AI image analysis for embryo selection to improve test accuracy and reproducibility.

Methods:

We established a developmental toxicity test protocol based on the zebrafish test. Gonadotropin was administered to induce ovulation, and in vitro fertilization was performed. Embryos were imaged at 5-6 and 6-7 h post-fertilization. AI image analysis was utilized to assess embryo viability. The test chemical was administered 24-48 h post-fertilization, and morphological changes were observed daily until day 8. Additionally, a time-lapse photography system was constructed to monitor embryonic development.

Results:

Out of 24 cultured embryos, 75% developed normally to the late tail bud stage or initial hatching stage, whereas 25% experienced developmental arrest or death. AI image analysis achieved high accuracy in classifying embryos, with overall accuracies of 92.0% and 92.9% for two learning models. The AI system demonstrated higher precision in the selection of viable embryos compared to visual inspection.

Conclusion:

The Iberian ribbed newt presents a viable alternative model for developmental toxicity testing, adhering to the 3Rs principles. The integration of AI image analysis substantially enhances the accuracy and reproducibility of embryo selection, providing a reliable method for evaluating developmental toxicity in pharmaceuticals.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article