Arabinoxylan from pearl millet bran: Optimized extraction, structural characterization, and its bioactivities.
Int J Biol Macromol
; : 135247, 2024 Aug 31.
Article
em En
| MEDLINE
| ID: mdl-39222787
ABSTRACT
Arabinoxylan (AX) from cereals and millets have garnered attention due to the myriad of their bioactivities. Pearl millet (Pennisetum glaucum) bran, an underexplored milling by-product was used to extract AX (PMAX) by optimized alkali-assisted extraction using Response Surface Methodology and Central Composite Design, achieving a yield of 15.96⯱â¯0.39â¯% (w/w) under optimal conditions (0.57â¯M NaOH, 117â¯g/mL solid-to-liquid ratio, 60⯰C, 4â¯h). Structural analysis revealed that PMAX was primarily composed of arabinose, xylose, glucose, galactose, and mannose (molar ratio 45.136.110.47.11.8), with a highly substituted (1â¯ââ¯4)-linked ß-D-xylopyranose backbone and a molecular weight of 794.88â¯kDa. PMAX displayed a significant reducing power of 0.617, metal chelating activity of 51.72â¯%, and DPPH, and ABTS radical scavenging activities (64.43 and 75.4â¯%, respectively at 5â¯mg/mL). It also demonstrated anti-glycation effects by inhibiting fructosamine (52.5â¯%), protein carbonyl (53.6â¯%), and total advanced glycation end products (77.0â¯%) formation, and reduced protein oxidation products such as dityrosine (84.7â¯%), kynurenine (80.2â¯%), and N'-formyl-kynurenine (50.0â¯%) at 5â¯mg/mL. PMAX induced the growth of Lactobacillus spp. in vitro and modulate gut microbiota in male Wistar rats by increasing Bacteroidetes and decreasing Firmicutes. These results provide a basis for further research on pearl millet arabinoxylan and its possible nutraceutical application.
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Ano de publicação:
2024
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Article