Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.
Nat Commun
; 15(1): 7717, 2024 Sep 09.
Article
em En
| MEDLINE
| ID: mdl-39251593
ABSTRACT
The ability to image tissues in three dimensions (3D) with label-free molecular contrast at the mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with optical sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3D molecular contrast in transparent and semi-transparent samples. We also created a computational pipeline for multivariate reconstruction to extract label-free spatial molecular information from Raman projection data. Using these tools, we demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and living tissue-engineered constructs and explanted native cartilage tissues. We show that there exists a favorable balance wherein employing Raman spectroscopy, with its advantages in live cell imaging and label-free molecular contrast, outweighs the reduction in imaging resolution and blurring caused by diffuse photon propagation. Thus, RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Imagens de Fantasmas
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Imageamento Tridimensional
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Imagem Molecular
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article