A dual sensitive fluorescence probe for the simultaneous visualization of hypochlorite and viscosity in living cells and zebrafish.

ABSTRACT

As vital microenvironmental indicators, hypochlorite and viscosity levels can be applied to diagnose diseases. Thus, real time and in situ monitoring of hypochlorite and viscosity has gained increasing attentions. A fluorescence probe named JDK was developed by using a novel acceptor of 2-(2-(5-(dimethylamino)thiophen-2-yl)vinyl)-1,3,3-trimethyl-3H-indol-1-ium fluorophore basing on FRET and TICT system. JDK possessed large Stokes shift, broad emission peak gap, prominent photostability and biocompatibility. JDK could track hypochlorite with rapid response time (within 1 min) and low detection limit (28.5 nM). Meaningfully, JDK was favor to append on mitochondria where physiological processes can be disrupted deriving from the abnormal concentrations of hypochlorite and viscosity. Successfully, JDK could visualize endogenous hypochlorite and viscosity in living cells and zebrafish.