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PCP-bε is a novel positive regulator of pollen germination in Arabidopsis thaliana.
Huang, Ziya; Guo, Li; Qin, Yidan; Gao, Qiguo.
Afiliação
  • Huang Z; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400716, China.
  • Guo L; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400716, China.
  • Qin Y; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400716, China.
  • Gao Q; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400716, China. Electronic address: gaoqg031@swu.edu.cn.
Biochem Biophys Res Commun ; 733: 150698, 2024 Nov 12.
Article em En | MEDLINE | ID: mdl-39298920
ABSTRACT
small cysteine-rich peptides play essential roles in different stages of the plant reproductive process. Pollen germination is a prerequisite for double fertilization and is directly related to seed formation and crop yield. However, the small cysteine-rich peptides that are involved in pollen germination remain to be identified. In this study, identification and phylogenetic analysis of PCP-Bε genes in sequenced Brassicaceae show that pollen coat protein B-class protein PCP-Bε gene is widespread in Arabidopsis and its high relatives, but lost in some Brassica species. Expression analyses display that AtPCP-Bε gene is expressed in Arabidopsis pollen. Arabidopsis PCP-Bε knockout mutants are generated by CRISPR/Cas9, Phenotypic analyses show that the absence of AtPCP-Bε obviously impairs in vitro pollen germination, but has no influence on pollen tube growth, which demonstrates that AtPCP-Bε is a novel positive regulator of pollen germination. It is speculated that AtPCP-Bε should interact with the receptor from pollen to perform its function. These findings are useful for further analysis on the molecular mechanism of pollen germination.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pólen / Arabidopsis / Germinação / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pólen / Arabidopsis / Germinação / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2024 Tipo de documento: Article