Spatial aspects of mannosyl phosphoryl retinol formation.
Biochim Biophys Acta
; 841(3): 299-305, 1985 Sep 06.
Article
em En
| MEDLINE
| ID: mdl-4027267
ABSTRACT
Rat liver microsomes catalyze the transfer of mannose from GDPmannose to both retinyl phosphate and dolichyl phosphate to form mannosylphosphorylretinol, mannosylphosphoryldolichol and GDP. The two reactions differ in term of reversibility. In fact, a 200-fold isotopic dilution of GDP[14C]mannose by unlabeled GDPmannose causes mannosylphosphoryldolichol labeling to disappear almost completely, while mannosylphosphorylretinol labeling remains at the same level. The same observation can be made if the mannose donor is removed by centrifugation and replaced by excess GDP; again mannosylphosphorylretinol is stable, but mannosylphosphoryldolichol drops down to one-third of its initial level, as expected for, respectively, a non-reversible and a reversible reaction. Placed in an aqueous medium, mannosylphosphorylretinol releases mannose 1-phosphate (beta configuration) whereas it is quite stable when kept in a membranous environment. These results strongly suggest that mannosylphosphorylretinol as soon as it is formed is segregated in such a way that it is no longer available to the back-reaction; the functional consequence of this segregation would be the possibility for mannosylphosphorylretinol to mannosylate some non-polar regions of certain protein chains.
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Base de dados:
MEDLINE
Assunto principal:
Açúcares de Poli-Isoprenil Fosfato
/
Fosfatos de Poli-Isoprenil
/
Vitamina A
/
Microssomos Hepáticos
/
Dolicol Monofosfato Manose
/
Fosfatos de Dolicol
/
Glicosídeos
/
Hexosiltransferases
/
Manosídeos
/
Manosiltransferases
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article