Characterization of bluetongue virus ribonucleic acid.
J Virol
; 5(1): 1-7, 1970 Jan.
Article
em En
| MEDLINE
| ID: mdl-4315158
ABSTRACT
An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Reoviridae
/
RNA Viral
Idioma:
En
Ano de publicação:
1970
Tipo de documento:
Article