Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b.
J Immunol
; 123(3): 1285-8, 1979 Sep.
Article
em En
| MEDLINE
| ID: mdl-469251
ABSTRACT
Antibody-dependent cytolytic effector lymphocytes are known to possess, in part, receptors for activated C3. Employing a model system consisting of 51Cr-labeled chicken erythrocytes and purified human peripheral lymphocytes, we investigated the effect of target cell bound C3b on antibody-dependent cellular cytotoxicity (ADCC). At concentrations of anti-target cell antibody too low to cause effective ADCC, target cell bound C3b cooperated with antibody to produce marked target cell lysis. In the presence of a 1/6.25 X 10(6) dilution of anti-chicken erythrocyte rabbit IgG, cell lysis increased from 20% to 65% by the attachment of 18,000 C3b molecules per cell. C3b-dependent enhancement of ADCC was dose dependent. It was augmented by attachment of activated properdin (P) to the C3b-bearing target cells.
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Base de dados:
MEDLINE
Assunto principal:
Complemento C3
/
Citotoxicidade Celular Dependente de Anticorpos
Idioma:
En
Ano de publicação:
1979
Tipo de documento:
Article