Restriction endonucleases from three strains of Haemophilus influenzae.
Sci Sin
; 23(11): 1435-42, 1980 Nov.
Article
em En
| MEDLINE
| ID: mdl-6262907
ABSTRACT
The restriction endonucleases, Hin P1 I1), Hin S1 I and Hin S2 I are isolated from three strains of Haemophilus influenzae respectively. By polymin P treatment, ammonium sulphate, precipitation and column chromatography on phosphocellulose and on heparin-Sepharose Hin P1 I is partially purified. No contaminating deoxyribonuclease activities have been detected in this purified enzyme preparation. The fact that the digestion patterns of Hin P2 I and Hha I on phage lambda, plasmids ColE1 an pBR 322 DNAs are identical that they are isoschizomers but theri splitting sites are different. The banding patterns of Hin S1 I and Hin S2 I are also the same as that of Hha I.
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Base de dados:
MEDLINE
Assunto principal:
Enzimas de Restrição do DNA
/
Haemophilus influenzae
Idioma:
En
Ano de publicação:
1980
Tipo de documento:
Article