Custom made C18 columns for the hydrophobic chromatography of cephalosporin C derivatives.
J Antibiot (Tokyo)
; 35(11): 1538-46, 1982 Nov.
Article
em En
| MEDLINE
| ID: mdl-6298166
ABSTRACT
"Hydrophobic chromatography", which is a variation of reverse phase chromatography, is applicable to the analysis of cephalosporin C derivatives, especially in fermentation broths. Unfortunately, there are no commercial C18 columns which are entirely suitable for this class of compounds. For this reason C18 columns were prepared by an in-situ bonding technique and were optimally designed for cephalosporin C derivatives. Mono-, di- and trifunctional octadecyl bonding agents were used with 10 microns silica of both 60 A and 100 A pore diameter. The best results were obtained with the difunctional agent, methyloctadecyldichlorosilane, and 100 A silica. "Endcapping" of residual silanol groups with a trimethylsilylation agent was optional, since good results were obtained with both a plain C18 column and one that was "endcapped".
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Base de dados:
MEDLINE
Assunto principal:
Cefalosporinas
Idioma:
En
Ano de publicação:
1982
Tipo de documento:
Article