A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF-stimulated tyrosine kinase.
FEBS Lett
; 153(2): 257-61, 1983 Mar 21.
Article
em En
| MEDLINE
| ID: mdl-6311615
ABSTRACT
The transforming proteins (p21) of Harvey and Kirsten sarcoma viruses threonine kinase activity, which phosphorylates threonine 59 of the p21 proteins themselves. A tridecapeptide Arg-Arg-Leu56-Asp-Thr-Thr59-Gly-Gln-Glu-Tyr-Ser-Ala66 containing residues 56-66 of p21 is phosphorylated solely on tyrosine by the epidermal growth factor (EGF)-stimulated tyrosine kinase of A431 cell membranes. Km-Values of 240 and 80 microM and Vmax values of 1.7 and 0.1 nmol.min-1.mg-1 were obtained in the presence and absence of EGF, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Proteínas Quinases
/
Transformação Celular Viral
/
Fator de Crescimento Epidérmico
/
Vírus do Sarcoma Murino de Kirsten
/
Vírus do Sarcoma Murino
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article